[Differential expression on cell wall formative genes in cell suspension cultures of Populus tomentosa Carr].
Identifieur interne : 004164 ( Main/Exploration ); précédent : 004163; suivant : 004165[Differential expression on cell wall formative genes in cell suspension cultures of Populus tomentosa Carr].
Auteurs : Xin-Guo Li [République populaire de Chine] ; Jin-Ju TianSource :
- Yi chuan = Hereditas [ 0253-9772 ] ; 2004.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Cellules cultivées (MeSH), Cellulose (biosynthèse), Cellulose (génétique), Feuilles de plante (cytologie), Feuilles de plante (enzymologie), Feuilles de plante (génétique), Glucosyltransferases (biosynthèse), Laccase (biosynthèse), Lignine (biosynthèse), Lignine (génétique), Paroi cellulaire (enzymologie), Paroi cellulaire (métabolisme), Populus (cytologie), Populus (enzymologie), Populus (génétique), Protéines végétales (biosynthèse), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Tiges de plante (cytologie), Tiges de plante (enzymologie), Tiges de plante (génétique).
- MESH :
- biosynthèse : Cellulose, Glucosyltransferases, Laccase, Lignine, Protéines végétales.
- cytologie : Feuilles de plante, Populus, Tiges de plante.
- enzymologie : Feuilles de plante, Paroi cellulaire, Populus, Tiges de plante.
- génétique : Cellulose, Feuilles de plante, Lignine, Populus, Tiges de plante.
- métabolisme : Paroi cellulaire.
- Analyse de profil d'expression de gènes, Cellules cultivées, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Cell Wall (enzymology), Cell Wall (metabolism), Cells, Cultured (MeSH), Cellulose (biosynthesis), Cellulose (genetics), Gene Expression Profiling (MeSH), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Glucosyltransferases (biosynthesis), Laccase (biosynthesis), Lignin (biosynthesis), Lignin (genetics), Plant Leaves (cytology), Plant Leaves (enzymology), Plant Leaves (genetics), Plant Proteins (biosynthesis), Plant Stems (cytology), Plant Stems (enzymology), Plant Stems (genetics), Populus (cytology), Populus (enzymology), Populus (genetics).
- MESH :
- chemical , biosynthesis : Cellulose, Glucosyltransferases, Laccase, Lignin, Plant Proteins.
- cytology : Plant Leaves, Plant Stems, Populus.
- enzymology : Cell Wall, Plant Leaves, Plant Stems, Populus.
- chemical , genetics : Cellulose, Lignin, Plant Leaves, Plant Stems, Populus.
- metabolism : Cell Wall.
- Cells, Cultured, Gene Expression Profiling, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant.
Abstract
With fine and granular suspension cultures established from P. tomentosa, differential expression of 12 genes related to cell wall formation including cell expansion, cellulose synthesis, lignin synthesis, etc, were investigated by reverse transcript PCR (RT-PCR) technique. Fine suspension cells showed high expression on 3 alpha-Exp genes with the highest alpha-Exp2 followed by alpha-Exp3 and alpha-Exp1 suggesting their strong cell expansion, while granular suspension cells only exist high expression on alpha-Exp3 indicating their later period of cell expansion or initial stage of secondary cell wall formation. Among the 6 genes relating to cellulose synthesis, both fine and granular cultures have high expression on CesA4 and CaSy1 and some expression on CesA1, CesA3-1 and CesA3-2, indicating their primary cell wall formation initiated, however a little higher expression on CesA3-1 and CesA3-2 in granular cells may reflect its more cellulose synthesis and more primary cell wall formation. The occurrence of Lac1 and Unk1 could not been detected in the 2 cell suspension cultures, showing no significant lignin biosynthesis and matural secondary cell wall formation.
PubMed: 15640084
Affiliations:
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083</wicri:regionArea><wicri:noRegion>Beijing 100 083</wicri:noRegion></affiliation></author><author><name sortKey="Tian, Jin Ju" sort="Tian, Jin Ju" uniqKey="Tian J" first="Jin-Ju" last="Tian">Jin-Ju Tian</name></author></analytic><series><title level="j">Yi chuan = Hereditas</title><idno type="ISSN">0253-9772</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (enzymology)</term><term>Cell Wall (metabolism)</term><term>Cells, Cultured (MeSH)</term><term>Cellulose (biosynthesis)</term><term>Cellulose (genetics)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glucosyltransferases (biosynthesis)</term><term>Laccase (biosynthesis)</term><term>Lignin (biosynthesis)</term><term>Lignin (genetics)</term><term>Plant Leaves (cytology)</term><term>Plant Leaves (enzymology)</term><term>Plant Leaves (genetics)</term><term>Plant Proteins (biosynthesis)</term><term>Plant Stems (cytology)</term><term>Plant Stems (enzymology)</term><term>Plant Stems (genetics)</term><term>Populus (cytology)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Cellules cultivées (MeSH)</term><term>Cellulose (biosynthèse)</term><term>Cellulose (génétique)</term><term>Feuilles de plante (cytologie)</term><term>Feuilles de plante (enzymologie)</term><term>Feuilles de plante (génétique)</term><term>Glucosyltransferases (biosynthèse)</term><term>Laccase (biosynthèse)</term><term>Lignine (biosynthèse)</term><term>Lignine (génétique)</term><term>Paroi cellulaire (enzymologie)</term><term>Paroi cellulaire (métabolisme)</term><term>Populus (cytologie)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Protéines végétales (biosynthèse)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Tiges de plante (cytologie)</term><term>Tiges de plante (enzymologie)</term><term>Tiges de plante (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cellulose</term><term>Glucosyltransferases</term><term>Laccase</term><term>Lignin</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cellulose</term><term>Glucosyltransferases</term><term>Laccase</term><term>Lignine</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plant Leaves</term><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Feuilles de plante</term><term>Paroi cellulaire</term><term>Populus</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cell Wall</term><term>Plant Leaves</term><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulose</term><term>Lignin</term><term>Plant Leaves</term><term>Plant Stems</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cellulose</term><term>Feuilles de plante</term><term>Lignine</term><term>Populus</term><term>Tiges de plante</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Cellules cultivées</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With fine and granular suspension cultures established from P. tomentosa, differential expression of 12 genes related to cell wall formation including cell expansion, cellulose synthesis, lignin synthesis, etc, were investigated by reverse transcript PCR (RT-PCR) technique. Fine suspension cells showed high expression on 3 alpha-Exp genes with the highest alpha-Exp2 followed by alpha-Exp3 and alpha-Exp1 suggesting their strong cell expansion, while granular suspension cells only exist high expression on alpha-Exp3 indicating their later period of cell expansion or initial stage of secondary cell wall formation. Among the 6 genes relating to cellulose synthesis, both fine and granular cultures have high expression on CesA4 and CaSy1 and some expression on CesA1, CesA3-1 and CesA3-2, indicating their primary cell wall formation initiated, however a little higher expression on CesA3-1 and CesA3-2 in granular cells may reflect its more cellulose synthesis and more primary cell wall formation. The occurrence of Lac1 and Unk1 could not been detected in the 2 cell suspension cultures, showing no significant lignin biosynthesis and matural secondary cell wall formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15640084</PMID><DateCompleted><Year>2006</Year><Month>01</Month><Day>04</Day></DateCompleted><DateRevised><Year>2016</Year><Month>10</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0253-9772</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>5</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Yi chuan = Hereditas</Title><ISOAbbreviation>Yi Chuan</ISOAbbreviation></Journal><ArticleTitle>[Differential expression on cell wall formative genes in cell suspension cultures of Populus tomentosa Carr].</ArticleTitle><Pagination><MedlinePgn>676-82</MedlinePgn></Pagination><Abstract><AbstractText>With fine and granular suspension cultures established from P. tomentosa, differential expression of 12 genes related to cell wall formation including cell expansion, cellulose synthesis, lignin synthesis, etc, were investigated by reverse transcript PCR (RT-PCR) technique. Fine suspension cells showed high expression on 3 alpha-Exp genes with the highest alpha-Exp2 followed by alpha-Exp3 and alpha-Exp1 suggesting their strong cell expansion, while granular suspension cells only exist high expression on alpha-Exp3 indicating their later period of cell expansion or initial stage of secondary cell wall formation. Among the 6 genes relating to cellulose synthesis, both fine and granular cultures have high expression on CesA4 and CaSy1 and some expression on CesA1, CesA3-1 and CesA3-2, indicating their primary cell wall formation initiated, however a little higher expression on CesA3-1 and CesA3-2 in granular cells may reflect its more cellulose synthesis and more primary cell wall formation. The occurrence of Lac1 and Unk1 could not been detected in the 2 cell suspension cultures, showing no significant lignin biosynthesis and matural secondary cell wall formation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xin-Guo</ForeName><Initials>XG</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Beijing Forestry University, Beijing 100 083, China. lixinguo@bjfu.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Jin-Ju</ForeName><Initials>JJ</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Yi Chuan</MedlineTA><NlmUniqueID>9436478</NlmUniqueID><ISSNLinking>0253-9772</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C095825">expansin protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C478648">cellulose synthase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.34</RegistryNumber><NameOfSubstance UI="C020570">1,3-beta-glucan synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Yi Chuan. 2004 Nov;26(6):897</RefSource></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="Y">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>1</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>1</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>1</Month><Day>11</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15640084</ArticleId><ArticleId IdType="pii">0253-9772(2004)05-0676-07</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Tian, Jin Ju" sort="Tian, Jin Ju" uniqKey="Tian J" first="Jin-Ju" last="Tian">Jin-Ju Tian</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Li, Xin Guo" sort="Li, Xin Guo" uniqKey="Li X" first="Xin-Guo" last="Li">Xin-Guo Li</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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